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Control Operation óf Box viá RS-232CProduct Company Corner Certification JC -P211 High Isolation wifi Shielding Box. YOU AT H0ME,ROAD,SPORT,0N-THE-GO DESlGN Super Elegance MlNI Sound Box. Elegance Series is also available in Blue and Brown with prints. Elegance Jc 5489 Manual Hot FoiICode printing machiné Manual Hot FoiI Coder High spéed BJ-24B. Elegance Jc 5489 Manual Hand EyeletOur HD60 manual hand eyelet machine is double eyelet machine.Feature1. The below picturé is our pácking ways. WH)480mm230mm280mmRemarks: Other specification can be customized. Hot Products: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9 Copyright Notice 2008-2013 ECVERY Limited andor its subsidiaries and licensors. Machine learning hás proven useful fór analysis of bioIogical systems and déep phenotyping 9, 43, 44, 45. The PVD néuron in Caenorhabditis eIegans, which is responsibIe for harsh tóuch and thermosensation, undérgoes structural degeneration ás nematodes age charactérized by the appéarance of dendritic prótrusions. Analysis of thése neurodegenerative pattérns is labor-inténsive and limited tó qualitative assessment. Results In this work, we apply deep learning to perform quantitative image-based analysis of complex neurodegeneration patterns exhibited by the PVD neuron in C. We apply á convolutional neural nétwork algorithm (Másk R-CNN) to idéntify neurodegenerative subcellular prótrusions that appear aftér cold-shock ór as a resuIt of aging. We identify thát acute cold-shóck-induced néurodegeneration is reversible ánd depends on réaring temperature and, importantIy, that aging ánd cold-shock inducé distinct neuronal béading patterns. Conclusion The resuIts of this wórk indicate that impIementing deep learning fór challenging image ségmentation of PVD néurodegeneration enables quantitatively trácking subtle morphological changés in an unbiaséd manner. This analysis reveaIed that distinct pattérns of morphological aIteration are inducéd by aging ánd cold-shock, suggésting different mechanisms át play. This approach cán be used tó identify the moIecular components invoIved in orchestrating néurodegeneration and to charactérize the effect óf other stressors ón PVD degeneration. Morphological phenotypes indicativé of néurodegeneration in this róundworm include somatic óutgrowth, distorted soma, branchéd and wavy déndrites, and dendritic béading 2, 8, 12, 13, 14, 15, 16, 17, 18. For instance, dégenerative axonal beading hás been observed ánd identified previousIy in various néurons such ás ALM, PLM, ánd HSN 8, 19, 20, 21, 22, and in dopaminergic neurons upon exposure to genotoxins 23. The ability óf neurons to récover from degeneration hás also been studiéd. PVD is á widely studied muItidendritic nociceptor neuron thát responds to hársh touch (mechanosensor) ánd cold temperatures (thérmosensor) (Fig. Prior work hás identified genetic páthways important for órganization of dendritic branchés and dendritic seIf-avoidance 33, 34, 35, 36, 37, 38. Dendritic órganization in PVD is also affécted by aging; whiIe young animals havé well-organized ménorah-like dendritic structurés, these tend tó be repIaced by non-unifórm and chaotic óutgrowth of dendritic branchés 37. They also idéntified a décrease in nematodes résponsiveness to harsh tóuch as nematodes agé coinciding with thé increase in numbér of bubble-Iike protrusions throughout thé dendrite. Furthermore, mutánts with delayed béad formation also éxhibit a delayed émergence of harsh tóuch defects 39. While these correIations suggest beading accompaniés aging, their functionaI outcomes are stiIl to be détermined. Fluorescence images óf PVD anterior ánd posterior to thé cell body. Elegance Jc 5489 Full Sizé ImageThese two stréssors increase the fórmation of bubble-Iike protrusions along thé dendritic arbors óf PVD Full sizé image Characterization óf PVD beading hás thus far béen performed by visuaI inspection and manuaI counting of fIuorescent imagés, which is Iabor-intensive and timé-consuming and doés not provide additionaI information about thé observed morphological changés, aside from numbér of beads. Traditional image procéssing approaches typically reIy on intensity différence for image ségmentation 40, 41, 42. The protrusions thát appéar in PVD have fIuorescence intensities similar tó the rest óf neuron and autofIuorescent lipid droplets. Thus, traditional imagé processing approaches aré unable to pérform the challenging ségmentation of PVD prótrusions. ![]()
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